We cleaned (removal of
roots and residues of seed coat) 60-day
old plants derived from the previous
experiments, produced in the treatment
with light, and placed them in test tubes
(150x25 mm) containing 15 mL of MS,
plus 30 g L-1 sucrose, solidified with
2.4 g L-1 Phytagel®, for 60 days, for
plant development. After this period,
we extracted 1.0 cm-long stems from
these plants to be used as explants
in this experiment.