An optimized protocol for regeneration of high yielding true-to-type plantations of S. acmella is developed by employing
leaf-disc explants of Spilanthes. By following this method, more than 155 plants can be produced per explants, in every 10 weeks. This rate of shoot multiplication has not been achieved till date in Spilanthes. Moreover, this is the first report, in which ploidy stability of in vitro regenerants has also been evalu-ated by flow cytometry. An interesting finding of this study is the identification and quantification of spilanthol, an anti-septic alkylamide, from in vivo and in vitro sources whereby in vitro leaves produced significantly higher amount of compounds (3294.36 ± 12.4 g/g DW) than that of field grown mother plant (2703.66 ± 9.6 g/g DW)
An optimized protocol for regeneration of high yielding true-to-type plantations of S. acmella is developed by employingleaf-disc explants of Spilanthes. By following this method, more than 155 plants can be produced per explants, in every 10 weeks. This rate of shoot multiplication has not been achieved till date in Spilanthes. Moreover, this is the first report, in which ploidy stability of in vitro regenerants has also been evalu-ated by flow cytometry. An interesting finding of this study is the identification and quantification of spilanthol, an anti-septic alkylamide, from in vivo and in vitro sources whereby in vitro leaves produced significantly higher amount of compounds (3294.36 ± 12.4 g/g DW) than that of field grown mother plant (2703.66 ± 9.6 g/g DW)
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