The sample of the test soil from raspberry field was placed
in a beaker with a different amount of sterile distilled water,
1/50, 1/100, 1/200 (g/ml water), and mixed with a mixer for 2
minutes and filtered through Whatman paper (Grade 1:11 μm).
From soil suspension 1 ml was taken and pipette into small
drops (40 drops from 1 ml; 10 in each plate) on the surface of
a 2% water agar (Duschefa, Netherlands) in Petri dishes. The
plates were cultured at 24°C for 18-24 hours. Determinations
of bacteria were recorded under a microscope observation.