An active inoculum from a thermophilic biogas plant
was needed. Inoculum from Vegger Biogasanlæg,
Aalborg, was used. Vegger Biogasanlæg is a biogas
plant in Denmarktreating primarily manure together
with some industrial organic waste and occasionally
organic household waste. The plant operates at 55 C.
The inoculum was transported in 25-liter containers
by a delivery service guarantying delivery within 48 h.
The temperature drops to ambient temperature during
delivery, but is always kept above the freezing point. In
order to readapt the inoculum to 55 C, ensure degradation
of easy degradable organic matter still present in
the inoculum and remove dissolved methane, the
inoculum was stored with an anaerobic headspace for
three days in the 55 C incubator