Depending upon how resistant starch is defined in vitro, retrograded amylopectin may contain different levels of resistant starch.
Compared with the enzyme susceptibility of native granular and
freshly gelatinized waxy maize starch, enzyme susceptibility of
retrograded waxy maize starch may be greatly reduced when
extensive retrogradation has occurred and it is incubated with
pancreatic a-amylase at 370C. Thus, influence of amylopectin
(retrogradation) on enzyme resistance may be observed only when
gelatinized starch has been stored in specific conditions.