The DPPH scavenging activity of the samples was measured using the modified method of Sun et al. (2008). 4.0 mL of 95%ethanol solution of DPPH (0.1 mM) was incubated with test samples (1.0 mL).
The reaction mixture was shaken well and incubated for 30 min at 33◦C in the dark and the absorbance of the resulting solution was read at 517 nm against a blank, the radical scavenging activity was measured as a decrease in the absorbance of DPPH.