Statistical Analysis
Pen means served as the experimental unit for statistical analysis. Data were initially analyzed using the GLM procedure of SAS (SAS Institute, 1991) as a completely randomized design (CRD), with factorial arrangements of phytase level, carbohydrase level, and the 2-way interactions between phytase, and xylanase levels. Mortality data were transformed to X +1 before analysis. Significant differences among treatments were determined at P < 0.05 using Tukey’s tests.
To estimate P equivalency of each enzyme combination, a quadratic equation was derived from dietary nPP (%) concentration and tibia ash (%) using a nonlinear regression model: Y = aX2+ bX + c, in which Y = the tibia ash as a percentage, and X = dietary nPP level (%) using data collected in a factorial experiment. Then this equation was solved using the tibia ash (%) data collected to calculate X values or inorganic P equivalency values of each of the treatments. To obtain the P equivalency of each enzyme and its combination, 0.15 (% of nPP level of basal diet) was subtracted from
the dietary P equivalence.
Statistical AnalysisPen means served as the experimental unit for statistical analysis. Data were initially analyzed using the GLM procedure of SAS (SAS Institute, 1991) as a completely randomized design (CRD), with factorial arrangements of phytase level, carbohydrase level, and the 2-way interactions between phytase, and xylanase levels. Mortality data were transformed to X +1 before analysis. Significant differences among treatments were determined at P < 0.05 using Tukey’s tests.To estimate P equivalency of each enzyme combination, a quadratic equation was derived from dietary nPP (%) concentration and tibia ash (%) using a nonlinear regression model: Y = aX2+ bX + c, in which Y = the tibia ash as a percentage, and X = dietary nPP level (%) using data collected in a factorial experiment. Then this equation was solved using the tibia ash (%) data collected to calculate X values or inorganic P equivalency values of each of the treatments. To obtain the P equivalency of each enzyme and its combination, 0.15 (% of nPP level of basal diet) was subtracted fromthe dietary P equivalence.
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