Serum was used for the estimation of albumin, creatinine, blood urea nitrogen (BUN), cholesterol and triglyceride levels. Pooled 24 h urine was evaluated for creatinine and albumin value. The esti- mation of the above mentioned parameters was carried out using biochemical kits (ACCUREX, Biomedical Pvt. Ltd). For the determi- nation of fluorescent AGE, a 96-plate spectrophotofluorimeter was employed. 100 ml of serum sample was placed in each well. Fluo- rescence intensity was read at 440 nm after excitation at 370 nm. Results were expressed as relative fluorescence intensity.