2.9. Kill-time analysis
The kill-time curve assay method was used to investigate the
bactericidal effects of the essential oil according to the technique
described by Joray, del Rollán, Ruiz, Palacios, and Carpinella (2011).
The cultivation with the essential oil was done the same as the
above MIC assay and controls containing only DMSO were simultaneously
run. At selected time intervals, samples from the test
culture were taken, serially diluted in sterile water, and plated in
Plate Count Agar (PCA) medium. All plates were then incubated for
24 h at 37 C, and CFU were counted