3. Results
3.1. Production of enzyme in a 300 L bioreactor
Several fermentor runs were carried out to optimize the production
of protease and lipase in a 300 L bioreactor, separately.
Parameters such as agitation rate, dissolved oxygen and inoculum
levels were optimized. Final yields for protease production showed
that the inoculum level (3%), dissolved oxygen levels greater than
resulted in 4586 U/mL of protease in 18 h (Fig. 1). Optimization of
various physiological factors revealed that the major controlling
factor for lipase production were inoculums density (3.0%), agitation
rate (250 rpm) and dissolved oxygen (D.O) levels at 40% saturations.
The lipase production from the present strain of B. subtilis
VVM