The starch content was calculated from the glucose obtained. The amylose content was analyzed according to the method developed by Sowbhagya and Bhattacharya (13). In brief, buckwheat flour was mixed with a solution (1N) of sodium hydroxide in ethanol, after which petroleum ether was added. After shaking, the layer of petroleum ether was aspirated off and carbon tetrachloride was added. The amylose content of the aqueous layer was determined by absorbance at 630nm, as measured by a Unicam PU 8630 spectrophotometer. Amylopectin content was estimated by subtracting the amylose content from the total starch content.