Antimicrobial assay
The antimicrobial assay was performed
by agar well diffusion method (3)
The disc was saturated with 100 μl of the
test compound, allowed to dry. For agar
well diffusion method, a well of 8 mm
diameter was prepared in the plate. 100 μl
of the extract was then introduced into the
well. The plate was incubated at 37oC for
24 hrs. and then observed for presence of
inhibition zone.