The significance of the Ames test has been clearly confirmed as a suitable primary test for the detection of potential mutagens and carcinogens, and since mid-1970s this assay has been routinely used as a screening assay to predict carcinogens (Maron and Ames, 1983).
The mutagenicity of KalmColdTM was determined using Salmonella reverse mutation assay both in the presence and absence of metabolic activation in S. typhimurium strains TA98 and TAMix at all the tested concentrations, i.e.,5000 lg/ml, 1510 lg/ml, 450 lg/ml, 140 lg/ml, 40 lg/ml and 10 lg/ml. The number of histidine revertants in negative and positive
controls was in accordance with the acceptable mean revertant values (data not shown).