After
verification of the system using red fluorescent protein the
authors displayed agarolytic enzymes on the cell surface for
agar utilization. Finally, a tobacco etch virus protease was
used to cleave the C-terminus of the displayed proteins for full
extracellular secretion. Sevastsyanovich et al. utilized the E.
coli serine protease Pet to develop a generalized
secretion system for a range of proteins varying in sizes and
structures, and including those multi-component proteins