2.2.1. Heat reflux extraction (HRE)
Rosemary leaves were submitted to reflux for two durations: 30 min and for 5 h. Extraction was done at boiling temperature (78 °C). To evaluate a potential effect of a fine dispersion in the solvent prior to extraction, the same experimental conditions were applied to rosemary leaves previously ground into the solvent during 3 min with a homogenizer (Ika T25 digital Ultra-Turrax, Germany). Each experiment was performed in triplicate.