The fluidized-bed bioreactor holds the advantage of being flexible to operate and easy to scale up and it seems to have the potential to be practically applied in large-scale biohydrogen production from organic wastes. For the sugary wastewater, enrichment in the presence of 2-bromoethanesulfonic acid resulted in lower H2 yields due to increased propionate production and decreased butyrate/acetate ratio. Adding Fe2+ helps improve the H2 production by 105% and speeds up the reaction. Adding l-cysteine enhances the H2 production by nearly 50%. Adding proper concentration of calcium ion could enhance bioH2 producing. Using activated carbon to remove bio-toxic inhibitors can improve the hydrogen yield. The rice straw hydrolysate mixing the feeding substrate with another high strength organic wastewater could enhance the productivity of biohydrogen in the continuous system. Sulfate concentration has a negative effect on biohydrogen production. An analysis of the economic benefits of this innovative reference commercial model prove its feasibility by using beverage wastewater and agricultural waste as feedstock which will enable a hydrogen-producing system to gain a maximum annual profit with an annual return rate of approximately 60% and 39% with Aspen Plus; 81% and 30% with local price evaluation. Some of the example from different sources, performance, and its operating conditions are reviewed and shown in Table 3.