Arrows,insoluble materials stacked on the well; arrowheads, Eap (60 kDa) and Efb (15
kDa), which were determined by N-terminal amino acid sequencing. (B) Cell
wall-anchored proteins were isolated from the indicated S. aureus strains after
lysostaphin treatment and centrifugation. The supernatants were analyzed by
SDS-PAGE. Molecular sizes are given at the left.