DPPH tests
The DPPH radical scavenging activity was evaluated accord-
ingto themethod described by Sharififar et al. (2009).1mL of
100 μM DPPHsolution in methanol was mixed with1 mLof
plant extract. The reaction mixture was incubated in the dark
for 20 min, and the optical density was recorded at 517nm
against the blank. For the control, 1mL of DPPH solution in
methanol was mixed with 1mL of methanol, andopticaldensity of solution was recorded after 20 min. IC50 values related
to the DPPH radical scavenging activity were calculated.