The elution gradient started at 10% B (0–1 min), linearly increased to 50% B (1–8 min), held at 100% B (8–9 min), decreased to 10% B (9–10 min), and held at 10% B (1–20 min).
The elution gradientstarted at 10% B (0–1 min), linearly increased to 50% B (1–8 min), heldat 100% B (8–9 min), decreased to 10% B (9–10 min), and held at 10%B (1–20 min).