An in vitro propagation protocol was standardized for commercially important cutflower
orchid Dendrobium Sonia((Fa. Orchidaceae)) ‘Earsakul.’ Stem nodal explants were used to
regenerate aseptic cultures in the culture establishment medium supplemented with auxins and
cytokinins. The culture establishment medium of ½ MS supplemented with 4 mg L-1 BA was
observed to give early bud break. In the shoot multiplication stage, treatment combinations of
2.0 mg L-1kinetin and 0.1 mg L-1 NAA was found to give earliest (11.00) shoot multiplication
and maximum numbers (4.66) of healthy shoots. The rooting media supplemented with 0.5 mg
L-1 NAA gave earliest rooting (19.6). Charcoal and brick pieces in 1:1 proportion gave highest
survival rate of 66.67 % at the planting out stage.