The fungal protease from Rhizomucor miehei is highly resistant
to the HPH process, with no changes in the PA and MCA when
processed at 2 g/100 mL at pressures of up to 190 MPa. Even multipasses
cause slight changes on the enzyme processed at this concentration.
On the other hand, for enzyme processed at high concentration
(20 g/100 mL) the process increased MCA, improved the
coagulation phase and the quality of the product (faster clotting
and higher consistency of the milk gel). Therefore, the HPH can be
used as a unitary operation to improve the competitiveness of this
fungal protease as milk coagulant.