because wheat gluten is partially soluble in acetic acid. Additionally, it is important to determine the effect of mixing on protein extractability by extracting proteins from both, dry mixes as well as dough. The amounts of proteins extracted from both, dry mixes or dough, decreased as the banana powder increased in the formulation, even though the ratio of protein:non-protein in the dry mix was kept the same by adding extra gluten. The amount of protein in the supernatant was determined and plotted against the amount of banana powder in the blends. A linear regression of the plotted data was used to show the linearity of the amount of proteins extracted from the dry mix and the dough as a function of banana powder content. The decrease in the extractable protein from the dry mixes followed a strait line with a good fit Y=36.9 X – 0.68 (R2 =0.97), whereas the dough had a poor fit with R2 = 0.40 and Y = 39.7 X – 0.83. This difference could be attributed to the formation of water-insoluble aggregates as part of the gluten-network formed during mixing while gluten transformed into a viscoelastic material. Considering the extra pro- tein added to the blends one might expect the amount of extract- able protein from control to be lower than the blends, but the data presented here showed otherwise. Except for the 10 and the 15% blends, the extractable protein from the remaining blends was lower than the control.
In an attempt to show changes in protein-fractions size, the ex- tracted proteins were analyzed using a SE-HPLC. The dough profile showed a typical wheat protein profile with different peak inten- sity according to the amount of protein in each sample. The first peak around 11.5 min represents the high MW glutenins followed by the low MW glutenins and finally the gliadins (Fig. 2). The SE- HPLC profile of the blends showed similar trends regarding the type of protein extracted. Although, there is similarity in the type of the extracted protein, some fractions showed more glutenins
because wheat gluten is partially soluble in acetic acid. Additionally, it is important to determine the effect of mixing on protein extractability by extracting proteins from both, dry mixes as well as dough. The amounts of proteins extracted from both, dry mixes or dough, decreased as the banana powder increased in the formulation, even though the ratio of protein:non-protein in the dry mix was kept the same by adding extra gluten. The amount of protein in the supernatant was determined and plotted against the amount of banana powder in the blends. A linear regression of the plotted data was used to show the linearity of the amount of proteins extracted from the dry mix and the dough as a function of banana powder content. The decrease in the extractable protein from the dry mixes followed a strait line with a good fit Y=36.9 X – 0.68 (R2 =0.97), whereas the dough had a poor fit with R2 = 0.40 and Y = 39.7 X – 0.83. This difference could be attributed to the formation of water-insoluble aggregates as part of the gluten-network formed during mixing while gluten transformed into a viscoelastic material. Considering the extra pro- tein added to the blends one might expect the amount of extract- able protein from control to be lower than the blends, but the data presented here showed otherwise. Except for the 10 and the 15% blends, the extractable protein from the remaining blends was lower than the control.In an attempt to show changes in protein-fractions size, the ex- tracted proteins were analyzed using a SE-HPLC. The dough profile showed a typical wheat protein profile with different peak inten- sity according to the amount of protein in each sample. The first peak around 11.5 min represents the high MW glutenins followed by the low MW glutenins and finally the gliadins (Fig. 2). The SE- HPLC profile of the blends showed similar trends regarding the type of protein extracted. Although, there is similarity in the type of the extracted protein, some fractions showed more glutenins
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