The evaluation of nutritional or functional components in grain products is an important feature for the
industry,especially when recent regulations require a correct nutrition labelling,valid during all the shelf
life of the product. For that reason, industry usually makes many efforts to develop simple and reliable
analytical methods that can be easily applied in any quality control laboratories for routine analysis.
Spectrofluorimetric analysis of thiamine and riboflavin are sensitive, but need specific equipment. A few
HPLC-UV methods have been described but they are less sensitive, and present difficulties due to
interfering compounds,particularly in complex food matrices, as grains and derivatives