3.4. Ion leakage
Plants harvested prior to cold acclimatisation, generally had a
higher ion leakage compared to cold acclimatised plants (Fig. 1).
Before cold acclimatisation a day length temperature interaction
was observed. The low growth temperature treatment, 12 h day
length had higher freezing tolerance compared to 24 h day. After
cold acclimatisation treatment there was also a significant lower
ion leakage in the low growth temperature treatments. As
observed before cold acclimatisation a temperature photoperiod
effect was seen: low growth temperature in combination with
short day regime resulted in even lower ion leakage compared to
long day, without cold acclimatisation.