After incubation at 35 ºC for 24 hours, the cultures were submitted
to a Gram coloring process. The cultures which showed short Gramnegative
bacilli were submitted to biochemical tests: indol, methyl red,
Voges-Proskauer and citrate. For the counting of mesophile bacteria,
there was the use of the deep sowing technique in standard counting
Agar (PCA). After the homogenization, the boards were the incubated
in an autoclave at 36 ± 2ºC for 48 hours