3.3 Ethanol and hydrogen production by separate hydrolysis and fermentation (SHF)
The maximum yield of ethanol (9.69 g/L) was achieved after 72 h of S. cerevisiae cultivation with no hydrogen production (Fig. 2a). S. cerevisiae, commercial ethanol-producing bacteria, has many
advantages over other known ethanol producing microorganisms. The most important are high ethanol
yields, ethanol tolerance, high robustness and high resistance to toxic inhibitors.
Lower production of ethanol (1.38 g/L) was observed after 72 h by E. aerogenes. However, E.
aerogenes yielded high hydrogen production (1,416 mL/L) after 12 h of cultivation (Fig. 2b). E.
aerogenes can convert various carbohydrates, such as sugars and sugar alcohols, to H2, ethanol, 2,3-
butanediol, lactate and acetate. Ito et al. [16] reported that E. aerogenes HU-101 mainly produces H2 and
ethanol with a minimal production of other by-products when glycerol was used as substrate.
However, both strains produced low ethanol and H2 production. It is necessary to further optimize
culture condition or improve production technique. Therefore, immobilized cell technology was selected
to increase the production of ethanol and H2 in this study.
3.3 Ethanol and hydrogen production by separate hydrolysis and fermentation (SHF)The maximum yield of ethanol (9.69 g/L) was achieved after 72 h of S. cerevisiae cultivation with no hydrogen production (Fig. 2a). S. cerevisiae, commercial ethanol-producing bacteria, has manyadvantages over other known ethanol producing microorganisms. The most important are high ethanolyields, ethanol tolerance, high robustness and high resistance to toxic inhibitors.Lower production of ethanol (1.38 g/L) was observed after 72 h by E. aerogenes. However, E.aerogenes yielded high hydrogen production (1,416 mL/L) after 12 h of cultivation (Fig. 2b). E.aerogenes can convert various carbohydrates, such as sugars and sugar alcohols, to H2, ethanol, 2,3-butanediol, lactate and acetate. Ito et al. [16] reported that E. aerogenes HU-101 mainly produces H2 andethanol with a minimal production of other by-products when glycerol was used as substrate.However, both strains produced low ethanol and H2 production. It is necessary to further optimizeculture condition or improve production technique. Therefore, immobilized cell technology was selectedto increase the production of ethanol and H2 in this study.
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