Screening Shoot Cultures For The Presence Of
Bacteria:
This method is used to eliminate contaminated explants
during initiation of material into culture and during
transfer of shoots to fresh medium. The essential steps
are as follows:
1. Prepare culture dishes containing bacterial
growth medium as indicated above.
2. Rub the bases of explants across the bacterial
growth medium before transferring explants to
plant tissue culture media.
3. Incubate the streaked dishes in the dark at
24°C.
4. After two days, visually evaluate the dishes for
the presence of bacteria.
5. Discard the subcultured explants that have
produced contaminated streaks.