Simultaneous saccharification and fermentation (SSF) exhibited
higher bioethanol productivity toward separation hydrolysis and fermentation (SHF). The optimal
condition of SSF was 5% of Kluyveromyces marxianus K21 inoculum at 40 C resulting in the maximum
ethanol concentration (48.98 g/L) and productivity (2.23 g/L/h) after 22 h of cultivation. The scaling up
experiment in a 5 L bioreactor demonstrated that K21 can still maintain its capability. After 20 h of cultivation,
43.78 g/L of ethanol (2.19 g/L/h of productivity) was achieved corresponding to a 94.2% theoretical
ethanol yield.