controlling heat stress (HS) is a global challenge for the dairy industry. in this work, an integrated metabolomics and lipidomics approach using 1H nuclear magnetic resonance (NMR) and ultra-fast LC-MS in combination with multivariate analyses was employed to investigat the discrimination fo plasma metabolic profiles between HS-free and HS lactating dairy cows.Here we provide the information about the acquiring and processing of raw data obtained by 1H NMR and LC-MS techniques. The data of present study are related to the research article "Identification of diagnostic biomarkers and metabolic pathway shifts of heat-stressed lactating dairy cows" in the journal of proteomics (Tian et al., J. Proteomics, (2015), doi:10.1016/j.jprot.2015.04.014).