Next,
a bacterial suspension of 2.0 × 108 cfu/mL was prepared in order
to determine the antimutagenic activity. Assays without metabolic
activation (−S9) were performed mixing 50 L of each
sample or standard solution with 450 L of phosphate buffer
(0.1M, pH 7.4), 100 L of bacteria suspension and 50 L of
mutagen solution (NPD 20 g/plate). After