2.5. Statistical analysis Statistical analysis was performed by using the software Graphpad Prism v6. Inhibitory concentration 50(IC50) with 95% confidence interval(95% CI) of Makona-GP and Mayinga-GP EBov neutralization were estimated by model of nonlinear regression fit with settings for log(inhibitor) vs normalize response curves. The p values neutralizing comparisons were calculated by non-linear fits obtained for Mayinga-GP and Makona-GP pseudotyped viruses using the Extra sum-of-squares F test.