Real Time PCR is a method that can be used to monitor amplicon formation throughout the PCR reaction providing
the ability to perform very sensitive, accurate and reproducible measurements of specific DNA present in a sample
(Bell and Ranford-Cartwright, 2002; Matsuu et al., 2005;Oyamada et al., 2005). In the present study, we development
and validated a highly sensitive qualitative Real Time PCR that amplifies a 125 bp fragment at the 3end of ITS2 of
the rDNA of B. canis subspecies. The overall prevalence rate of B. canis vogeli by the Real Time PCR (9.9%) was higher than that found by direct examination of blood smears (0.8%),confirming the higher sensitivity of the method.