The secondary seed culture was produced by
inoculating the primary seed culture into the 200 mL of
modied terric broth medium and incubated for overnight
as used in primary seed culture. This overnight
grown secondary seed culture was inoculated into the
bioreactor containing presterilized 4-L modied terric
broth medium for fermentation. Fermentation process
was carried out in a fully automated NBS fermentor
(Bioow-3000; New Brunswick Scientic) with 5 L
working volume. BioCommand plus software was used for
operation of the fermentor as per the instructions manual
from the manufacturer.