TRIzol reagent (Sigma Life Science, Madrid, Spain) and RNeasy Lipid
Tissue mini kits (QIAGEN, Madrid, Spain) were used for the LT muscle
and SF, respectively, according to the manufacturer's instructions. Furthermore,
5 samples of 200 mg from the intestine (jejunum) and the
liver were also extracted using TRIzol reagent and were used to confirm
primer specificity and tissue specific gene amplification.