2.6.1.2. Hydrogen peroxide (H2O2) scavenging activity
To evaluate the hydrogen peroxide (H2O2) scavenging activity of the extracts, the H2O2 solution was prepared in phosphate buffer (pH 7.4) and then 0.6 mL of 40 mM H2O2 solution was mixed with 0.1 mL of different concentrations (10–500 μg/mL) of the extracts. Absorbance of hydrogen peroxide at 230 nm was determined after 10 minutes against a blank solution containing phosphate buffer without hydrogen peroxide. Ascorbic acid (5–250 μg/mL) was taken as the reference compound [19].