Finally, the antifungal activity of the unique volatile compounds
identified from the headspace of all D. hansenii cultures in the
presence of the mould (2-methyl-1-propanol, 3-methyl-1-butanol
and 2-methyl-1-butanol) was evaluated according to the above
described mouth-to-mouth method. However, in this experiment
15 ml of each volatile compound were placed in the lower position
in an empty Petri dish. The effect of a mix of the three selected
volatile compounds on P. verrucosum Pv21 growthwas also studied.
For controls, 15 ml of sterile deionized water were used. The
experiment was done in triplicate.