Improving plantlet survival rate and the proper transplant substrates selection are
keys for plantlets development in the greenhouse and even their potential yield
achievement. Chen [2007] demonstrated that if regenerated plantlets were trans-cultured
in hormone free MS medium after in rooting induction medium, rooting rate, root length
as well as stem height would have higher values. And after attempting different substrates,
he found that substrates containing only coco shell powder gave the highest
transplantation survival rate (95.2%) under greenhouse conditions (21 ± 2C, 80–90%
relative humidity). At the end, it must be emphasized that in order to obtain high quality
transplantable plantlets from in vitro culture, multiculture for rooting and plantlet development
is necessary.