Zymography is an electrophoretic technique for the detection of hydrolytic enzymes, based on the substrate repertoire of the enzyme. Three types of zymography are used; in gel zymography, in situ zymography and in vivo zymography[1]
Zymography is an electrophoretic technique,
based on SDS PAGE for measuring enzyme activity
• The technique is particularly useful for analyzing
the proteinase composition of complex biological
samples
• Zymography is also widely used to study various
aspects of matrix metalloproteinase (MMP)