The goal of this study is to explorethe potential of the novel pgi-gene knockout strain, GALG20,for pDNA production in a bench-scale bioreactor. The parentalstrain, MG1655endArecA and the common laboratory strain,DH5, were also studied and used as benchmarks. As a first step,the performance of the production strains was characterized inshake flask experiments and in bioreactors operated in batchmode (Table 1). While in shake flask GALG20 produced 10 timesmore