A two-stage process, composed of growth under nutrient-rich conditions followed by cultivation under
nitrogen starvation and controlled conditions of phosphate, light intensity, aeration, and carbon sources
was applied for lipid production by the green alga Chlorella vulgaris. Using conditions without addition of
nitrogen, 2 mg/L PO4-P, light intensity of 100 lmol/m2/s and 0.25 vvm of air, about 43% of dry cell weight
accumulated as lipids after 12 h, which equates to a lipid productivity of 77.8 mg/L/d. In a medium containing
5 mg/L NO3-N and 2 mg/L PO4-P, and at a light intensity of 100 lmol/m2/s and 0.25 vvm of 2%
CO2, about 53% of dry cell weight consisted of lipids after 24 h, representing a lipid productivity of
77.1 mg/L/d. The low amount of nutrients, moderate aeration and light intensity were helpful for increasing
lipid productivity.
A two-stage process, composed of growth under nutrient-rich conditions followed by cultivation under
nitrogen starvation and controlled conditions of phosphate, light intensity, aeration, and carbon sources
was applied for lipid production by the green alga Chlorella vulgaris. Using conditions without addition of
nitrogen, 2 mg/L PO4-P, light intensity of 100 lmol/m2/s and 0.25 vvm of air, about 43% of dry cell weight
accumulated as lipids after 12 h, which equates to a lipid productivity of 77.8 mg/L/d. In a medium containing
5 mg/L NO3-N and 2 mg/L PO4-P, and at a light intensity of 100 lmol/m2/s and 0.25 vvm of 2%
CO2, about 53% of dry cell weight consisted of lipids after 24 h, representing a lipid productivity of
77.1 mg/L/d. The low amount of nutrients, moderate aeration and light intensity were helpful for increasing
lipid productivity.
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