A standard curve of pixel intensity was generated for the cholesterol
standards using Photoshop and used to quantify the amount of
cholesterol in the lipid droplet samples. To determine the levels of cholesterol relative to TAG in lipid droplets, a second TLC plate loaded
with lipid droplet samples in triplicate, separated via the same single
solvent system and the amounts of TAG and PL was quantified by the
above described method of GC/MS analysis