a b s t r a c t
In this study, enzyme inhibitory and antioxidant activity of the ethanol extracts of Centella asiatica (L.)
Urban from Turkey and India was examined and the results were compared to those of the standardized
extract of the plant obtained from China. Inhibitory activity of these three extracts was screened
against four enzymes; acetylcholinesterase (AChE), butyrylcholinesterase (BChE), tyrosinase (TYRO), and
lipoxygenase (LOX) using ELISA microplate reader. Antioxidant activity of the extracts was determined
in vitro using 2,2-diphenyl-1-picrylhydrazyl (DPPH), metal-chelation, and ferric-reducing antioxidant
power (FRAP) assays. The extracts showed a notable inhibition against BChE and TYRO, while the extracts
from Turkey and India displayed lower activity in the antioxidant assays. HPLC analysis indicated that
the Turkish plant sample was found to be richest in p-hydroxy-benzoic acid, whilst the Indian sample
contained chlorogenic acid in the most abundant amount. -Copaene was the dominant compound in
the essential oil of the plant sample from Turkey.