After optimizing the medium composition, experiments were
conducted to evaluate the effect of different concentrations (0.0–4.0 mg l−1) of BAP or Kin
along with 0.1 mg l−1 of naphthalene acetic acid (NAA) on shoot bud induction. The cultures
were incubated at 28±2 °C under diffused (10–20 μmol m−2 s−1) light for 16 h day−1
photoperiod for 3–4 days and then exposed to 50–60 μmol m−2 s−1 for 16 h day−1 photoperiod.