This was clinically unacceptable due to the potential of overestimate HbA2 by CAE+densitometry resulting in false-positive diagnosis of βTT as well as a greater number of cases with borderline HbA2 requiring molecular work-up (Table 1). CAE+densitometry overestimated HbA2 (i.e., yielded values > 3.3% as compared to lower results on HPLC) in 13 cases overall, yielding a false-positive βTT rate of 10.6%.