Spirulina platensis, planktonic blue–green algae, is gaining increasing attention because of its nutritional and medicinal
properties. This microalgae contains phycobiliproteins (phycocyanin and allophycocyanin). Previous reports from our laboratory
have shown that a protean extract of S. platensis is a potent free-radical scavenger (hydroxyl and peroxyl radicals) and inhibits
microsomal lipid peroxidation. The aim of this study was to purify and characterize phycocyanin of S. platensis. Besides, we tried
to demonstrate that one of the main components responsible for this antioxidant activity is a biliprotein phycocyanin. For this
purpose, we studied the antioxidant activity of different fractions obtained during the phycocyanin purification process, through
the scavenger activity of hydroxyl radical. We also observed that an increase in phycocyanin content was related to an increase
in the antioxidant activity in different fractions, and therefore phycobiliprotein phycocyanin is the component mainly responsible
for the antioxidant activity. © 2001 E´ ditions scientifiques et me´dicales Elsevier SAS
Spirulina platensis, planktonic blue–green algae, is gaining increasing attention because of its nutritional and medicinalproperties. This microalgae contains phycobiliproteins (phycocyanin and allophycocyanin). Previous reports from our laboratoryhave shown that a protean extract of S. platensis is a potent free-radical scavenger (hydroxyl and peroxyl radicals) and inhibitsmicrosomal lipid peroxidation. The aim of this study was to purify and characterize phycocyanin of S. platensis. Besides, we triedto demonstrate that one of the main components responsible for this antioxidant activity is a biliprotein phycocyanin. For thispurpose, we studied the antioxidant activity of different fractions obtained during the phycocyanin purification process, throughthe scavenger activity of hydroxyl radical. We also observed that an increase in phycocyanin content was related to an increasein the antioxidant activity in different fractions, and therefore phycobiliprotein phycocyanin is the component mainly responsiblefor the antioxidant activity. © 2001 E´ ditions scientifiques et me´dicales Elsevier SAS
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