For this reason, hydrophobic interaction has became the most popular method for lipase
immobilization, and various hydrophobic materials have been used to achieve this, such as Accurel EP-100, Octyl agarose, PSLGmodified PPMM, and polysulfone nanofibrous membranes [17–20]. However, better immobilized lipase systems with characteristics of being more effective, more durable, and less expensive are
still of great demand to facilitate the commercialization of enzymatic biodiesel synthesis processes.