2.2. Experimental design
L. rohita fingerlings (mean bodyweight 11.1 ± 0.07 g) were
acclimatised to laboratory conditions in 500-L quarantine tanks at
26 ± 2 C for 2 weeks and were fed a basal diet. Approximately 20%
of the water in all tanks was exchanged daily and 100% of the water
was exchanged weekly. Basic physioechemical parameters of the
water were measured weekly [26]. Oxygen and ammonia concentrations
were 6.1e7.3 mg L1 and 0.03e0.06 mg L1, respectively,
and pH ranged 7.0e8.0 throughout the study period.