Nowadays, b2-agonists are abused illegally as ‘‘lean meat agents’’ for food-producing animals, and
cause increasing food-safety accidents in some countriesDue to their hazard to the human health,
‘‘lean meat agents’’ are banned in most countries and required to be routinely monitored. We herein
report a disposable electrochemiluminescent immunosensors array for near-simultaneous assay of
multiple b2-agonist residues in swine urine, by using ractopamine and salbutamol as the models. In this
investigation, a screen-printed carbon electrodes array was assembled and acted as the substrate of the
immunosensors array Then the immunosensors array was constructed by site-selectively immobilizing
the antigens of ractopamine and salbutamol on the working electrodes of array.Then the immunosensors array was constructed by site-selectively immobilizing
the antigens of ractopamine and salbutamol on the working electrodes of array.The limits
of detection for ractopamine and salbutamol were 8.5 and 17 pg/mL, respectively, which were much
lower than those of the most previous reports. Compared with other routine methods based on
chromatography and ELISA, this method is more suitable for screening of multiple b2-agonists in
quantities of samples, owing to its merits of low cost, user-friendliness and high throughput, and shows
great promise in food safety and agonist surveillance.