The study is focused on different scenarios taking into account several parameters affecting the overall efficiency of the process of production and use. The analysis takes into account the possible biomass supply chains, the different types of biomass exploitable as primary source and different technologies for conversion. The different stages from the production, through the up-grading, up to the availability at the tank have been evaluated. The latest Italian regulation in the distribution field is also taken into account. By a Well-To-Wheel analysis an estimation of the primary energy savings and greenhouse gas emissions reduction is performed, in comparison to the use of methane from fossil source.