The expression profiles of 375 miRNAs in serum exosomesamples isolated
with UC (n=5) and EQ (n=5) methods were determined by qRTPCR
using Exiqon Human panel I assays. The average numbers of detected
exosomal miRNAs above the detection limit (Ct b 35) were 128 (range
100 to 160) and 93 (range 87 to 108) for EQ and UCmethods, respectively.
According to raw Ct values, the average miRNA detection threshold
levels were 1.7 cycles lower in EQ samples, indicating that the total
exosomal miRNA quantity isolated from an equal amount of serum is
higher in EQ processed samples. The most abundant miRNAs detected
by qRT-PCR were similar between the isolation methods (Table 1).